Dr. Andre Nel from UCLA will be speaking at 3 pm on November 7, 2012 at the Hostler Auditorium, Health Sciences Center
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Nanomaterial Toxicity Testing in the 21st Century: Use of High Throughput Approaches to Understand Oxide Nanoparticle Toxicity
André E. Nel, M.D./PhD
Professor of Medicine and Chief of NanoMedicine, UCLA School of Medicine
Director, UC Center for Environmental Implications of Nanotechnology
Director, UCLA Center for Nanobiology and Predictive toxicology
Abstract: To develop safety screening commensurate with the level of nanotechnology growth and development, high throughput screening (HTS) approaches and predictive toxicological paradigms are being developed by the UC Center for the Environmental Implications of Nanotechnology (CEIN) and the UCLA Center for NanoBiology and Predictive Toxicology. This platform allows the assessment of potentially hazardous nanomaterial properties at the nano/bio interface that can be used to make predictions of adverse health effects outcomes in vivo. This talk will delineate the use of a multi-parametric HTS assay for oxidative stress to demonstrate its ability to predict the acute inflammatory effects of 24 oxide nanoparticles in the lung based on semiconductor properties, particle dissolution and the ability to generate ROS based on band gap overlap with cellular redox potential. I will demonstrate the use of in silico and statistical tools to establish in vitro hazard ranking and structure-activity relationships that can be used for in vivo hazard ranking. This approach allows the prioritization of pulmonary toxicity testing in animals. I will also demonstrate that zebra fish HTS can be used for environmental decision-making about the hazard of metal oxides premised on shedding of toxic metal ions that interfere with a metalloprotease responsible for embryo hatching. This allows hazard ranking that is being prioritize ecosystems studies in CEIN.
1. Lin, S., et al. High Content Screening in Zebrafish Speeds up Hazard Ranking of Transition Metal Oxide Nanoparticles. ACS Nano (2011).
2. Xia, T.,et al. Comparison of the abilities of ambient and manufactured nanoparticles to induce cellular toxicity according to an oxidative stress paradigm. Nanoletters 6: 1794-1807 (2006).
3. George, S., et al. Use of a Rapid Cytotoxicity Screening Approach to Engineer a Safer Zinc Oxide Nanoparticle through Iron Doping. ACS Nano. 4:15-29 (2010).
4. Zhang, H., et al. Use of metal oxide nanoparticle band gap to develop a predictive paradigm for acute pulmonary inflammation based on oxidative stress. ACS Nano. In Press (2012).
5. Nel, A.E., et al. Toxic potential of materials at the nanolevel? Science. 311: 622-627 (2006).
Dr. André Nel is a Professor of Medicine and Chief/founder of the Division of NanoMedicine at UCLA. He is the Director of the UC Center for the Environmental Impact of Nanotechnology (CEIN) and the UCLA Center for Nano Biology and Predictive Toxicology which are housed in the California NanoSystems Institute (CNSI) at UCLA. Dr. Nel served as Chair of an NIH Study Section, has been nominated as a Distinguished Foreign Professor by the Chinese Academy of Science, a peer-selected member of Best Doctors of America, recipient of the John Salvaggio Memorial Award recognizing his outstanding service to the specialty and science of Allergy and Immunology, and has represented the NIH and State Department in establishing cooperative research agreements with Japan, the Chinese Academy of Sciences and Russia. He is associate editor of ACS Nano and his chief research interests are nanomedicine, nanotechnology environmental health and safety, and nanobiology.
Recent Selected Publications:
1. Lin, S., Zhao, Y., Xia, T., Meng, H., Ji, Z., Liu, R., George, S., Xiong, S., Wang, X., Zhang, H., Pokhrel, S., Lutz Mädler, L., Damoiseaux, R., Lin, S., and Nel, A.E. High Content Screening in Zebrafish Speeds up Hazard Ranking of Transition Metal Oxide Nanoparticles. ACS Nano 5 (9):7284-95 (2011).
2. Nel, A.E., Xia, T., Meng, H., Wang, X., Lin, S., Ji, Z., and Zhang, H. Nanomaterial Toxicity Testing in the 21st Century: Use of a Predictive Toxicological Approach and High Throughput Screening. Acc. Chem. Res. Epub ahead of print (2012).
3. George, S., Lin, S., Ji, Z., Thomas, C.R., Li, L., Mecklenburg, M., Meng, H., Wang, X., Zhang,H., Xia, T., Hohman, J.N., Lin, S., Zink, J.I., Weiss, P.S., Nel, A.E. Surface Defects on Plate-Shaped Silver Nanoparticles Contribute to its Hazard Potential in a Fish Gill Cell Line and Zebra Fish Embryos. ACS Nano. 6(5):3745-59 (2012).
4. Zhang, H., Ji, Z., Xia, T., Meng, H., Low-Kam, C., Liu, R., Pokhrel, S., Lin, S., Wang, X., Liao, Y.P., Wang, M., Li, L., Rallo. R., Damoiseaux, R., Telesca, D., Mädler, L., Cohen, Y., Zink, J.I., Nel, A.E. Use of Metal Oxide Nanoparticle Band Gap To Develop a Predictive Paradigm for Oxidative Stress and Acute Pulmonary Inflammation. ACS Nano 6(5):4349-68 (2012).
5. Wang, X., Xia, T., Duch, M.C., Ji, X., Zhang, H., Li, R., Sun, B., Lin, S., Meng, H., Liao,Y., Wang, M., Song, T.,Yang, Y., Hersam, M.C., and Nel, A.E. Pluronic F108 Coating Decreases the Lung Fibrosis Potential of Multiwall Carbon Nanotubes by Reducing Lysosomal Injury. Nano Lett 12(6):3050-61 (2012).